ELISA is the most widely used technique in enzyme immunoassay. Since its advent in 1960s-1970s, ELISA kit has been recognized and accepted by the world's scientific research workers, and it has been greatly promoted in the field of biochemical development such as in Europe, the United States and Asia.

ELISA test is a highly sensitive, specific and reproducible experimental diagnostic method. Because it is stable, easy to save and easy to operate, and the results are more objective, ELISA kit has been widely used in immunological tests in various fields.

However, when researchers have to choose a new ELISA kit, there are always some difficult choices to make, considering there are thousands of ELISA references available on the market. So here are some suggestions based on my experience with long term projects, which are five key steps you need to consider when selecting the most appropriate immuno-assay kit:

1.Clearly understand what kind of protein to detect;

2.Find out which gene that specifically encodes the protein is best homologous to the other species;

3.To find a kit for detecting proteins from these species

4.Consult the ELISA suppliers what type of antibodies are used in the kit: polyclonal antibody or monoclonal antibody? What is the monoclonal antibody against the antigenic determinant?

5.Make the own decision on which to buy the kit.