Second, when sandwich ELISA are used to galactomannan antigen in fungal cell wall, the antigens can release into the blood. Sensitivity and specificity of this test are high and the sensitivity improves as time goes. It is meaningful to early diagnosis of deep fungal infections for the fungal antigens test can get continuous monitoring of high-risk patients.

Third, capsular polysaccharide antigen of Cryptococcus neoformans can be tested through latex agglutination test or ELISA. If the antigens are detected in cerebrospinal fluid, it can be diagnosed as cryptococcus encephalitis. But one thing have to be noticed that RF need to be removed. If not, the test will be incorrect.